You could do the dilutions in one step, or you might have to do serial dilutions. ... How about a 1:100 dilution followed by a 1:2 dilution? (1) Add ...
2-fold-dilution
Explanation: What you suggest is 10-fold dilution. 2-fold would be 1x, 1/2x, 1/4x in your notation. However, .... The two main approaches are optimization of primer concentration and/or ... In this example the efficiency is calculated using both 10-fold and 2-fold dilution ... 3925e8d270
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